MICROBIOLOGY Essay Example | Topics and Well Written Essays - 500 words

MICROBIOLOGY - Essay Example IgD: - IgD has a serum concentration of 30Â µg/ml and constitutes about 0.2% of total serum immunoglobulin. It is the major membrane bound immunoglobulin expressed by mature B – cells and is involved in the activation of B – cells by antigen. c. In immediate hypersensitivity antigen binds to IgE on mast cells and basophils. This cross linking leads to degranulation with the release of biologically active substances contained in the granules. 5. a. In the ouchterlony method both antigen and antibody diffuse radially from wells toward each other, thereby establishing a concentration gradient. As equivalence is reached, a visible line of precipitation forms. This simple technique is an effective qualitative tool for determining the relationship between antigens and the number of different Ag-Ab systems present. ELISA Test: - An enzyme conjugated to an antibody reacts with a colorless substrate to generate colored reaction product. Enzymes used include alkaline phosphates, horseradish peroxides. ELISA test is used to detect the presence of antibodies and antigen. RIA: - RIA is a competitive binding assay in which fixed amounts of antibody and radio labeled antigen react in presence of unlabelled antigen. The labeled and unlabelled antigens compete for limited binding sites on the antibody. After reaction, the antigen is separated into free and bound fractions and their radioactive counts measured. The concentration of the unlabelled (test) antigen can be calculated from the ratio of the bound and total antigen labels, using standard dose response curve. 6. It is difficult to develop a natural system of Bacterial Taxonomy using the traditional technique of Taxonomy because bacteria are generally very small and have simple shapes, and hence their classification by shape is not an easy task. The revolution in Genomics will affect Taxonomy because with the coming of

Discuss the role of the courage of children in both Little Woman and Essay

Discuss the role of the courage of children in both Little Woman and Treasure Island - Essay Example â€Å"Treasure Island reflects a deep anxiety about the power imbalance that complicates the adult author-child reader relationship†¦ Stevenson worries that the authors of adventure stories aim to indoctrinate and exploit youngsters like his impressionable boy hero† (Gubar, 2009, pp126-127). Furthermore, both Treasure Island and Little Women were written in the 19th century, which has been labelled the â€Å"Golden Age† of children literature (Gubar, 2009). In considering the novels in their historical context, Gubar’s observation underlines the changing approach of authors to children’s literature towards the end of the nineteenth century in portraying childhood and growing up. A central element of this is through the subversion of societal norms associated with children with the use of strength. For example, in Treasure Island, the novel’s central protagonist Jim Hawkins relays the journey of pirates and hidden treasure and the narrative is rich with action of childhood adventure, which is infused with adult themes such as morality, alcoholism and murder (Jones, 2003). Moreover, it is submitted that Jim’s character arguably fuses the universal quest for adventure conventionally associated with childhood with serious issues impacting child development, development in growing up. This is exemplified by the characterisation of Jim as a boy man in taking the Hispaniola to get the buried treasure: â€Å"the scheme had an air of adventure that inspired me, and the thought of the water breaker beside the fore companion doubled my growing courage† (Stevenson, 1883, p.196). Similarly, Alcott’s â€Å"Little Women† also subverts the contextual norms associated with children growing up, which is arguably attributable to Alcott’s difficult childhood (Clark 2005, p.213). Indeed, Alcott utilises the familiarity of domesticity associated with girls and subverts this to underline the complex issues impacting children’s lives, which was contextually